LOCUS       40924_13340        3757 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCRB15 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Tsuchida Y, Kimura S, Suzuki N, Inui M, Yukawa H.
  TITLE     Characterization of a 24-kb plasmid pCGR2 newly isolated from 
            Corynebacterium glutamicum
  JOURNAL   Appl. Microbiol. Biotechnol. 87 (5), 1855-1866 (2010)
  PUBMED    20552356
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Inui M, Suzuki N, Noburyu R, Yukawa H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2008) Contact:Masayuki Inui Research Institute of 
            Innovative Technology for the Earth, Molecular Microbiology and 
            Biotechnology Group; 9-2 kizugawadai, Kizugawa, Kyoto 619-0292, 
            Japan URL :http://www.rite.or.jp/
REFERENCE   3  (bases 1 to 3757)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2010"; volume: "87"; issue: "5"; 
            pages: "1855-1866"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JUL-2008) Contact:Masayuki Inui Research Institute of Innovative
            Technology for the Earth, Molecular Microbiology and Biotechnology 
            Group; 9-2 kizugawadai, Kizugawa, Kyoto 619-0292, Japan URL 
            :http://www.rite.or.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(14..385)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(405..452)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     protein_bind    673..694
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        709..739
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    747..763
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     771..787
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    797..853
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(857..873)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1199..1787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    1840..3724
                     /note="derived from Corynebacteriumt glutamicum cryptric
                     plasmid pCG1"
ORIGIN
        1 tagcttatcc tcagtcctgc tcctctgcca caaagtgcac gcagttgccg gccgggtcgc
       61 gcagggcgaa ctcccgcccc cacggctgct cgccgatctc ggtcatggcc ggcccggagg
      121 cgtcccggaa gttcgtggac acgacctccg accactcggc gtacagctcg tccaggccgc
      181 gcacccacac ccaggccagg gtgttgtccg gcaccacctg gtcctggacc gcgctgatga
      241 acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag tcccgggaga
      301 acccgagccg gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg
      361 gaacggcact ggtcaacttg gccatgatgg ccctcctata gtgagtcgta ttatactatg
      421 ccgatatact atgccgatga ttaattgtca aaacagcgtg gatgggatat caaggcagtt
      481 attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg
      541 gcagaaattc agcttggccc agtgccaagc tccaatacgc aaaccgcctc tccccgcgcg
      601 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      661 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      721 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      781 ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
      841 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      901 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      961 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgagcttctt
     1021 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1081 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1141 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1201 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1261 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1321 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1381 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1441 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1501 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1561 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1621 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1681 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1741 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1801 tcttttctac ggggtctgac gctcagtgga actagatctg gaaccgttat ctgcctatgg
     1861 tgtgagcccc cctagagagc ttcaagagca atcagcccga cctagaaagg aggccaagag
     1921 agagacccct acggggggaa ccgttttctg cctacgagat ggcacattta ctgggaagct
     1981 ttacggcgtc ctcgtggaag ttcaatgccc gcagacttaa gtgctctatt cacggtctga
     2041 cgtgacacgc taaattcaga catagcttca ttgattgtcg gccacgagcc agtctctccc
     2101 tcaacagtca taaaccaacc tgcaatggtc aagcgatttc ctttagcttt cctagcttgt
     2161 cgttgactgg acttagctag tttttctcgc tgtgctcggg cgtactcact gtttgggtct
     2221 ttccagcgtt ctgcggcctt tttaccgcca cgtcttccca tagtggccag agcttttcgc
     2281 cctcggctgc tctgcgtctc tgtctgacga gcagggacga ctggctggcc tttagcgacg
     2341 tagccgcgca cacgtcgcgc catcgtctgg cggtcacgca tcggcggcag atcaggctca
     2401 cggccgtctg ctccgaccgc ctgagcgacg gtgtaggcac gctcgtaggc gtcgatgatc
     2461 ttggtgtctt ttaggcgctc accagccgct tttaactggt atcccacagt caaagcgtgg
     2521 cgaaaagccg tctcatcacg ggcggcacgc cctggagcag tccagaggac acggacgccg
     2581 tcgatcagct ctccagacgc ttcagcggcg ctcggcaggc ttgcttcaag cgtggcaagt
     2641 gcttttgctt ccgcagtggc ttttcttgcc gcttcgatac gtgcccgtcc gctagaaaac
     2701 tcctgctcat agcgtttttt aggtttttct gtgcctgaga tcatgcgagc aacctccata
     2761 agatcagcta ggcgatccac gcgattgtgc tgggcatgcc agcggtacgc ggtgggatcg
     2821 tcggagacgt gcagtggcca ccggctcagc ctatgtgaaa aagcctggtc agcgccgaaa
     2881 acgcgggtca tttcctcggt cgttgcagcc agcaggcgca tattcgggct gctcatgcct
     2941 gctgcggcat acaccggatc aatgagccag atgagctggc atttcccgct cagtggattc
     3001 acgccgatcc aagctggcgc tttttccagg cgtgcccagc gctccaaaat cgcgtagacc
     3061 tcggggttta cgtgctcgat tttcccgccg gcctggtggc tcggcacatc aatgtccagg
     3121 acaagcacgg ctgcgtgctg cgcgtgcgtc agagcaacat actggcaccg ggcaagcgat
     3181 tttgaaccaa ctcggtataa cttcggctgt gtttctcccg tgtccgggtc tttgatccaa
     3241 gcgctggcga agtcgcgggt cttgctgccc tggaaatttt ctctgcccag gtgagcgagg
     3301 aattcgcggc ggtcttcgct cgtccagcca cgtgatcgca gcgcgagctc gggatgggtg
     3361 tcgaacagat cagcggaaaa tttccaggcc ggtgtgtcaa tgtctcgtga atccgctaga
     3421 gtcatttttg agcgctttct cccaggtttg gactgggggt tagccgacgc cctgtgagtt
     3481 accgctcacg gggcgttcaa catttttcag gtattcgtgc agcttatcgc ttcttgccgc
     3541 ctgtgcgctt tttcgacgcg cgacgctgct gccgattcgg tgcaggtggt ggcggcgctg
     3601 acacgtcctg ggcggccacg gccacacgaa acgcggcatt tacgatgttt gtcatgcctg
     3661 cgggcaccgc gccacgatcg cggataattc tcgctgccgc ttccagctct gtgacgacca
     3721 tgctagatct gtcgaacgga agatcacttc ggatatc
//
